Hioec cell
WebbDysregulation of mammalian target of rapamycin (mTOR) signaling contributes to head and neck squamous cell carcinoma (HNSCC) tumorigenesis and progression. In the current study, we tested the anti-HNSCC cell activity by GDC-0349, a selective ATP-competitive inhibitor of mTOR. We showed that GDC-0349 inhibited proliferation of established and … Webb一、细胞名称:HOEC 二、二、中文名称:人口腔上皮细胞 三、生长特性: 贴壁 悬浮 半悬浮半贴壁 四、 HOEC (人口腔上皮细胞) 生长条件: 五、组成: 六、 HOEC (人口腔上皮细胞) 接收后的操作流程与注意事项 1. 细胞收到后建议在培养箱稳定4小时左右再依据细胞密度,换液培养或传代。 2. 如果细胞为贴壁细胞,而收到时呈悬浮或者部分悬浮的状 …
Hioec cell
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Webb14 maj 2012 · HIOEC and OSCC cells plated overnight were stimulated with LPS (1 μg/ml) for 8 h and then stained as described in Materials and Methods. a to c, control cells … WebbHIOEC and OSCC cell lines (5 × 105 ml-1) were pretreated with or without 1 μg/ml LPS for 4 h, then stimulated with 1 μg/ml cisplatin for 24 h. The cells were stained with Annexin V and PI and...
Webb1 mars 2013 · HIOEC cells were maintained in defined keratinocyte-SFM medium (Gibco-BRL, Carlsbad, CA, USA) and the other cells were maintained in DMEM supplemented with 10% heat-inactivated fetal bovine serum (Gibco). All cells were cultured in a humidified atmosphere of 5% CO 2 at 37°C. Webb目的研究人乳头状瘤病毒HPV16 E6/E7永生化口腔上皮细胞的上皮-间叶变(EMT),以及发生EMT细胞的生物学特征.方法相差显微镜、微分干涉显微镜观察HPV16 E6/E7永生化口腔上皮细胞系HIOEC细胞形态学改变;透射电镜观察细胞间桥粒形成情况; RT-PCR比较正常口腔上皮细胞和HIOEC细胞中β-catenin、上皮钙黏蛋白(E ...
WebbHuman oral epithelial cell line HIOEC were pur-chased from Shanghai iCell Bioscience Inc. (China) and exclusively kept in an incubator at 37°C and 5%CO 2. HNSCC cell line CAL-27 (Shanghai iCell Bioscience Inc., China) was grown in DMEM medium (Servicebio, Wuhan, China) with 10% fetal bovine serum (FBS, Webb7 feb. 2024 · HIOEC cells were seeded at 1 × 10 5 cells per 100 mm plate (one plate per biological replicate), grown to 90–100% confluency (refreshed medium every other day) and subjected to 1.2 mM Ca 2+ in culture medium for 3 days. Cell were washed with ice-cold PBS (Hyclone) and fixed with 1% paraformaldehyde (PFA) for 10 min at room …
Webb1 maj 2008 · The HIOEC and HB96 cell lines from a cellular carcinogenesis model of OSCC was as described previously.27 OSCC cell line of CAL27 was purchased from …
Webb2 maj 2024 · HIOEC cells were cultured in defined Keratinocyte-SFM (Gibco). All cells were incubated in a humidified atmosphere containing 5% CO 2 at 37 °C. Immunohistochemistry (IHC) staining For IHC staining, the procedures were as described previously [ 23 ]. coup de chance film woody allenWebbCOX-2PGE2激活JAKSTAT3信号通路调控人口腔癌KB细胞增殖侵袭和转移. COX-2 对 KB 细胞增殖、侵袭和 EMT 的促进作用ꎮ 结论:COX-2 / PGE2 激活 JAK / STAT3 信号通路促进. 人口腔癌 KB 细胞增殖、侵袭和 EMTꎮ. 【 关键词】 COX-2ꎻ PGE2ꎻ JAK / STAT3ꎻ 口腔癌ꎻ 增 殖ꎻ 侵 袭 ... coup de griffe meaningWebbHIOEC cells were maintained in culture medium supplemented with 1.2mM Ca2+ for 3 days, and then were disassociated with 0.25% trypsin-EDTA. Cultured HEPM cells … coup de grace wineWebbThe cell line was named HIO615. Western blot analysis showed HIO615 expressed HPV16 E6 and E7 protein. HIOC were positive for cytokeratin, tonofibril and … coup de main meaningWebbHIOEC (Q54889587) From Wikidata. Jump to navigation Jump to search. cell line. Human Immortalized Oral Epithelial Cells; edit. Language Label Description Also known as; English: HIOEC. cell line. Human Immortalized Oral Epithelial Cells; Statements. instance of. cell line. 1 reference. stated in. Cellosaurus release 43. retrieved. 10 October ... coup de pousse celeo scram mp3 downloadWebb1 sep. 2024 · 此外HIOEC细胞形态由椭圆形变成纺锤形;从TEM结果显示,EV处理后的上皮细胞中核质内陷,出现多个核仁,此为口腔癌前病变的典型特征。 表明OSCC-EV增强了HIOEC的EMT,促进其发生癌前转化。 4. miR-let-7c/p53/PTEN通路参与了正常上皮细胞的癌前转化 OSCC-EV下调了HIOEC细胞中p53和PTEN的蛋白质水平,由于EV可通过传 … coup de gras new worldWebbPurpose: To investigate the influence of HPV16 E5 on E6 and E7 gene in human oral immortalized epithelial cells (HIOEC). Methods: The pLEGFP-E5 was transferred into … coup der holland job