WebDescription pEF-DEST51 is a 7.5 kb vector derived from pEF6/V5-His and adapted for use with the Gateway ™ Technology. It is designed to allow high-level, constitutive expression in a variety of mammalian hosts. Features pEF-DEST51 contains the following elements: • Human elongation factor 1α WebpEF6/Myc-His To propagate and maintain the pEF6/Myc-His vectors, we recommend resuspending each vector in 20 μl sterile water to prepare a 1 μg/μl stock solution. Store the stock solution at -20°C. Use the stock solution to transform a recA, endA E. coli strain like TOP10F´, DH5αF´, or equivalent.

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WebThermo Fisher mammalian expression pef6 v5 his topo plasmid vector Mammalian Expression Pef6 V5 His Topo Plasmid Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. WebpEF6 V5-His TOPO® TA Vector Kit pEF6/V5-His TOPO® TA Vector Kit, included in the MembranePro™ Functional Protein Expression Kit and also available separately (see page 29), contains the reagents listed below. Upon receipt, store the vector kit at –20°C. Reagent Composition Amount pEF6/V5-His-TOPO® vector 10 ng/µL plasmid DNA 20 µL lake county school days off

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WebpEF6/V5-His (Search Vector Database) Backbone manufacturer Invitrogen Backbone size w/o insert (bp) 5840 Total vector size (bp) 6531 Vector type Mammalian Expression Selectable markers Blasticidin Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Unknown … WebApr 30, 2024 · PCR products were inserted into pEF6/V5-His B vector (Invitrogen) at EcoRV sites using In-Fusion HD Cloning Kit (TaKaRa) or NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, MA, USA). The plasmids of dNlgns were modified by replacing V5-His tag with FLAG tag. Therefore, we obtained the following five plasmids: pEF6-dNlgn1-FLAG, … WebJun 2, 2014 · WT and mutant SOX11 cDNAs were PCR amplified and cloned into the pEF6/V5-His B mammalian expression vector (Life Technologies) using the In-Fusion PCR Cloning Kit (Clontech Laboratories), and also ... lake county school calendar 2022-23